| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Woodhead, Bryan (21)
|
MRWISC |
23.03 |
22.88 |
-0.15 |
| 2 |
Galarza, Matthew G (16)
|
MREAST |
25.16 |
24.93 |
-0.23 |
| 3 |
Crane, Brian A (14)
|
MREAST |
24.24 |
25.14 |
0.90 |
| 4 |
Kwon, Steven (13)
|
MRYMID |
25.56 |
25.64 |
0.08 |
| 5 |
Ma, Jonathan Y (13)
|
MREAST |
25.75 |
25.80 |
0.05 |
| 6 |
Torres, Cristian A (15)
|
MRYMID |
25.83 |
25.86 |
0.03 |
| 7 |
Rattazzi, John P (17)
|
MRSSL |
26.06 |
26.11 |
0.05 |
| 8 |
Koch, Alexander H (13)
|
MRCFB |
25.73 |
26.17 |
0.44 |
| 9 |
Lewis, Garfield (17)
|
MRYMID |
26.24 |
26.46 |
0.22 |
| 10 |
Saraf, Akaash (14)
|
MREAST |
27.47 |
27.11 |
-0.36 |
| 11 |
Crosbie, Kyle T (14)
|
MRSSL |
27.77 |
27.15 |
-0.62 |
| 12 |
Miller, Alexander (13)
|
MRYMID |
27.41 |
27.83 |
0.42 |
| 13 |
Kiesel, Garrett W (13)
|
MREAST |
27.52 |
27.96 |
0.44 |
| 14 |
Sasso, Thomas B (13)
|
MREAST |
28.00 |
28.27 |
0.27 |
| 15 |
Kornfeld, Myles A (15)
|
MREAST |
28.40 |
28.43 |
0.03 |
| 16 |
Vales, Tanner C (13)
|
MREAST |
29.34 |
28.73 |
-0.61 |
| 17 |
Uriarte, Alvin (15)
|
MRYMID |
|
29.33 |
- |
| 18 |
Fuchs, Ethan (15)
|
MRRYE |
31.80 |
29.55 |
-2.25 |
| 19 |
Fairweather, Sheldon (15)
|
MRYMID |
29.62 |
29.56 |
-0.06 |
| 20 |
Macchetto, Thomas (13)
|
MRCFB |
30.06 |
29.63 |
-0.43 |
| 21 |
Heisler, Lucas D (17)
|
MRYMID |
29.68 |
30.73 |
1.05 |
| 22 |
Dennis, Sean (13)
|
MRSSL |
34.10 |
31.11 |
-2.99 |
| 23 |
Sternberg, David (14)
|
MRCFB |
32.03 |
31.34 |
-0.69 |
| 24 |
Fang, Ethan (13)
|
MREAST |
31.39 |
32.17 |
0.78 |
| 25 |
Radovanovich, Anthony (13)
|
MRCFB |
33.16 |
33.16 |
0.00 |
|
McTeigue, Reese A (16)
|
MRYMID |
31.13 |
NS |
- |
|
Walsh, Zachary R (13)
|
MRYMID |
31.17 |
NS |
- |
|
Haffner, Jackson (14)
|
MRRYE |
29.90 |
NS |
- |